Species Reactivity : Canine (Canis familiaris; Dog)
UniProt : N/A
Abbreviation : IgG4
Alternative Names : N/A
Application : ELISA
Range : 1.23-100 ?g/mL
Sensitivity : 0.47 ?g/mL
Intra-AssayCV : ?4.5%
Inter-AssayCV : ?8.3%
Recovery : 0.93
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate IgG4 in samples. An antibody specific for IgG4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyIgG4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for IgG4 is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IgG4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : Immunoglob µLin G (IgG) is a monomeric immunoglob µLin, built of two heavy chains ? and two light chains. Each IgG has two antigen binding sites. It is the most abundant immunoglob µLin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglob µLins in humans. IgG molec µLes are synthesised and secreted by plasma B cells. IgG antibodies are predominately involved in the secondary antibody response, which occurs approximately one month following antigen recognition, thus the presence of specific IgG generally corresponds to maturation of the antibody response. This is the only isotype that can pass thro µgh the human placenta, thereby providing protection to the fetus in utero. Along with IgA secreted in the breast milk, residual IgG absorbed thro µgh the placenta provides the neonate with humoral immunity before its own immune system develops.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).