AKT1 antibody

Volume : 100 µg
Purification : Immunogen affinity purified
Form : liquid
Purity : 95% as determined by SDS-PAGE
Host : Rabbit
Clonality : polyclonal Ab
Clone ID :
Isotype : IgG
Storage : PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20? for 12 months (Avoid repeated freeze / thaw cycles.)
Background : The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs thro µgh phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1 which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. M µLtiple alternatively spliced transcript variants have been found for this gene.
Immunogen : v-akt murine thymoma viral oncogene homolog 1
Aliases : AKT, AKT1 PKB, PKB ALPHA, PRKBA, Protein kinase B, Proto oncogene c Akt, RAC, RAC ALPHA, RAC PK alpha
Observed MW : 60 kDa
Uniprot ID : P31749
Reactivity : Human, Mouse, Rat
Application : ELISA, WB, IHC, IF
Recommended dilution : WB : 1 : 500 - 1 : 2000; IHC : 1 : 50 - 1 : 100; IF : 1 : 50 - 1 : 100
Gene ID : 207
Research Area : Metabolism, Cardiovasc µLar, Immunology, Developmental biology, Neuroscience, Stem Cells