AKT1 (Ab 473) Antibody

Volume : 100 µL
Clone Number :
Aliases : AKT 1 antibody; AKT antibody; AKT1 antibody; AKT1_HUMAN antibody; C AKT antibody; cAKT antibody; MGC99656 antibody; PKB alpha antibody; PKB antibody; PKB-ALPHA antibody; PRKBA antibody; Protein Kinase B Alpha antibody; Protein kinase B antibody; Proto-oncogene c-Akt antibody; RAC Alpha antibody; RAC antibody; Rac protein kinase alpha antibody; RAC Serine/Threonine Protein Kinase antibody; RAC-alpha serine/threonine-protein kinase antibody; RAC-PK-alpha antibody; v akt murine thymoma viral oncogene homolog 1 antibody; vAKT Murine Thymoma Viral Oncogene Homolog 1 antibody
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : P31749
Immunogen : Peptide sequence around aa. 471~475 (Q-F-S-Y-S) derived from Human Akt.
Raised in : Rabbit
Species Reactivity : Human, Mouse, Rat
Tested Applications : ELISA, WB, IHC; WB : 1 : 500-1 : 1000, IHC : 1 : 50-1 : 200
Background : General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI3K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), ins µLin and ins µLin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating ins µLin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates ins µLin-stim µLated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the ins µLin-induced activation of glycogen synthase.

Baudhuin LM, et al. (2004) FASEB J Feb; 18(2) : 341-3.
Min YH, et al. (2004) Cancer Res; 64(15) : 5225-31.
Feng J, et al. (2004) J Biol Chem; 279(34) : 35510-7.
Ayala G, et al. (2004) Clin Cancer Res; 10(19) : 6572-8.
Lungu AO, et al. (2004) J Biol Chem; 279(47) : 48794-800.
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conj µgates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Conj µgate : Non-conj µgated
Buffer : Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form : liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : AKT1
Research Areas : Epigenetics and Nuclear Signaling?Neuroscience?Cancer;Metabolism;Signal transduction