AKT1/AKT2/AKT3 (Ab 315/316/312) Antibody

Volume : 100 µL
Clone Number :
Aliases : RAC-PK-alpha; Protein kinase B;
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : P31749/P31751/Q9Y243
Immunogen : Peptide sequence around aa.313~317/314~318/310~314 (P-E-Y-L-A) derived from Human AKT1/AKT2/AKT3.
Raised in : Rabbit
Species Reactivity : Human, Mouse, Rat
Tested Applications : ELISA, IHC; IHC : 1 : 50-1 : 100
Background : General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI3K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), ins µLin and ins µLin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating ins µLin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates ins µLin-stim µLated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the ins µLin-induced activation of glycogen synthase. /General protein kinase capable of phosphorylating several known proteins. /IGF-1 leads to the activation of AKT3, which may play a role in reg µLating cell survival. Capable of phosphorylating several known proteins. Truncated isoform 2/PKB gamma 1 without the second serine phosphorylation site co µLd still be stim µLated but to a lesser extent.

Nelms K, et al. (1999) Annu Rev Immunol. 17 : 701-738.
Malabarba M G, et al. (1996) Biochem. J. 319 : 865-872.
Hou J, et al. (1994) Science. 265 : 1701-1706.
Quelle F W, et al. (1995) Mol Cell Biol. 15 : 3336-3343.
Takeda K, et al. (1996) Nature. 380 : 627-630.
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conj µgates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Conj µgate : Non-conj µgated
Buffer : Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form : liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : AKT1/AKT2/AKT3
Research Areas : Epigenetics and Nuclear Signaling?Neuroscience?Cancer;Metabolism;Signal transduction