Acetyl P53 (Lys386) Antibody

Volume : 100 µL
Host : Rabbit
Clonality : polyclonal Ab
Isotype : IgG
GeneName : TP53
GeneID : 7157
Uniprot : P04637
Specificity : Acetyl-P53 (Lys386) Antibody detects endogenous levels of P53 only when acetylated at Lys386.
Immunogen : A syntheVolumed peptide derived from human P53 around the acetylation site of Lys386.
Storage : Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Reactivity : Human,Rat
Prediction :
Application : IHC,IF/ICC,ELISA(peptide)
Dilution : IHC 1 : 50-1 : 200, IF/ICC 1 : 100-1 : 500, ELISA(peptide) 1 : 20000-1 : 40000
Conj µgate : Unconj µgated
RRID : AB_2847060
An Introduction to Blocking Peptides : A superior strategy for validation of antibody binding is the use of blocking peptides. In a Western Blotting assay, our blocking peptide was validated to block the signal of this antibody. This ensures the site-specificity of an antibody, including phosphor-antibodies. In the case of the phosphor- antibodies, the antibodies can and will only recognize the phosphorylated target protein at the corresponding site, not the non-phosphorylated target protein or target protein phosphorylated at a different site.
Cat# Blocking Peptide : AF3746-BP
Des# Blocking Peptide : Acetyl-P53 (Lys386) Blocking peptide
Blocking Peptide Price : $549 CAD